Biological Sciences, Vol. 4, Issue 1, Jun  2020, Pages 1-8; DOI:

Behavioral and Histopathological Alterations in (Oreochromis Niloticus) Caused by Cypermethrin Pesticide Exposure

Biological Sciences, Vol. 4, Issue 1, Jun  2020, Pages 1-8.


Haram Hassan Abbas Bakhiet 1* , Amna Ahmad 1 , Emad Abaker 1 , Khalda Yahya 1

1 Department of Fisheries and Wildlife Science College of Animal Production Science and Technology, Sudan University of Science and Technology, Khartoum North ,Sudan

Received: 1 January 2020; Accepted: 29 April 2020; Published: 17 May 2020

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This study aimed to investigate the effects and toxicity of short term exposes of insecticide Cypermethrin on (Oreochromis niloticus) fish tissues (liver and gills), and fish behavior. Oreochromis niloticus fish were obtained from the fish Hatchery of the department of fisheries and wildlife science. (30) Fish of both sexes were used in this experiment. average weight was (50 -100 g.) fish were divided in to five groups and subjected to Cypermethrin insecticide solution in 4 dosages The first group was exposed to 0.0001ppm, the total amount of solution was 3 ml Cypermethrin insecticide / 30 liter water (D) , second group was exposed to 0.0002ppm, by 6 ml / 30 liter water (B), third group was exposed to 0.0003ppm by 9 ml / 30 liter water(E), The fourth group was exposed to 0.0004ppm, by 12ml/ 30 liter water (A) Results of histology indicates several kinds of damages in all studied organs, in liver showed Focal areas of necrosis, dilation and thrombosis formation in central vain and ruptured hepatocytes and vacuolation, pyknosis and hemorrhages, gills shows sloughing of secondary lamellae and proliferation and damage in the epithelium of gill filaments and congestion in blood vessels of gills  filaments and atrophy of secondary lamella Many behavioral observation were notice, rapid swimming and lose of balance were observed in different dosages.


Water Pollution, Insecticide, Fish, Behavior, Health, Risk, Environment


© 2017 by the authors. Licensee International Technology and Science Press Limited. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


[1] AlaaG.M.O.; Abd-El –Baset, M.AbdE.R.; KhalidY.A.; AliG. GadEl-Rab, AEnzymatic and histopathologic biomarkers as indicators of aquatic pollution in fishesNatural Science, 20102(11), 1302-1311.

[2] ALI, S.S. Morphological and biochemical hazards caused by some organochlorinated insecticides on blood and liver of rat. PhD thesis, Department of Zoology, University of the Punjab, Lahore1989.

[3] BabuV.; Mariadoss S.; Elif , P.C.; ErhanU. Histopathological Changes in the Gill and Liver Tissues of Freshwater Fish, Cirrhinus mrigala Exposed to Dichlorvos. Brazilian Archives of Biology and  Technology, 2009, 52, 5-9.

[4] Bharat, B.P.; Hongray, H.J.; Theresia M.; Selvanayagam M. Histopathology of gill, liver, muscle and brain of (Cyprinus carpio) exposed to sublethal concentration of lead and cadmium. African Journal of Biotechnology2011, 10(57), 12218-12223.

[5] Camargo, M.; Martinez, C. Biochemical and physiological biomarkers in Prochilodus lineatus submitted to in situ tests in an urban stream in southern Brazil. J Environmental Toxicology and Pharmacology, 2006, 21, 61-69.

[6] Camargo, M.M.; Martinez, C. Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotropical Ichthyology, 2007, 5, 327-336.

[7] Fewtrell and colford. Water Sanitation and hygiene: Intervention and diarrhea a systematic review and Metal. Analysis water supply and sanitation board the World BankAvailable online: http: // bank (accessed on 31 March 2020).

[8] GrahamR.; Scott, A.; KatherineA.; SlomanB. The effects of environmental pollutants on complex fish behavior: integrating behavioral and physiological indicators of toxicityAquatic Toxicology, 2004, 68(11), 369-392.

[9] Hamilton, R.M. Discharge of pesticides to the rivers, Mole and Taw, their accumulation in fish flesh and possible effects on fish stocks. J. Fish Biol., 1985, 27(5), 139-149.

[10] Kontek, B.; Globowski, K.; Paradowski, S. Chlorinated hydrocarbons in blood of agricultural workers with occupational exposure to pesticides. Polski. Tygot. Lek., 1976, 31(7)265-267.

[11] LouisA.; Helfrich E. Pesticides and Aquatic Animals: A Guide to Reducing Impacts on Aquatic Systems, Produced by Communications and Marketing, College of Agriculture and Life Sciences, Virginia Polytechnic Institute and State University, 2009; pp. 420-013.

[12] Merriam WebsterAvailable online: https://www.merriam /pollution (accessed on 31 March 2020).

[13] Mohamed, M.; Osman, M.; Potter, T.; Levin, R. Lead and cadmium in Nile River water and finished drinking water in greater Cairo, Egypt. Environment International, 1998, 2(24), 767-772.

[14] Morgan, D.P.; Lin, L.I. Blood organochlorine pesticide concentrations, clinical hematology and biochemistry in workers occupationally exposed to pesticides. Arch. environ. Contam. Toxicol, 1978, 2(7), 423-428.

[15] Olojo, E.; Olurin, K.; Mbaka, G.; Oluwemimo, A. Histopathology of the gill and liver tissues of the african catfish Clarias gariepinus exposed to lead. African Journal of Biotechnology, 2005, 1(4)117-122.

[16] Palmar, O. Algae and water pollution the identification significance and Control of algae in water supplies and in polluted water “castle House publication Laliberte, 1994.

[17] Pickerjng, Q.H.; Henderson, C.; Lenke, A.E. The toxicity of organophosphorus insecticides to different species of warm water fishes. Trans. Amer. Fish Soc, 1962, 91(2)175-184.

[18] Sabullah, M.K.; Ahmad, S.A.; Shukor, M.Y.; Gansau, A.J.Syed, M.A.; Sulaiman, M.R.; Shamaan, N.A. Heavy metal biomarker: Fish behavior, cellular alteration, enzymatic reaction and proteomics approachesInternational Food Research Journal, 2015, 22(2)435-454.

[19] Silva, H.C.; Medina H.S.G.; Fanta E.; Bacila, M. Sub-lethal effects of the organophosphate Folidol600 (Methyl Parathion) on Callichthys callichthys (Pisces: Teleostei). Compo Biochem. Physiol, 1993, 105(2), 197-201.

[20] Soufy, H.; Soliman, M.; El-Manakhly, E.; Gaafa, A. Some biochemical and pathological investigations on monosex Tilapia following chronic exposure to carbofuran pesticides. Global Veterinaria, 2007, 1(3), 45-52.

[21] Vieira, L.R.; Gravato, C.; Soares, A.M.V.; Morgado, F.; Guilhermino, L. Acute effects of copper and mercury on the estuarine fish Pomatoschistus microps: Linking biomarkers to behavior. Chemosphere, 2009, 76(9), 1416-1427.

[22] YangY.G.; Yang, G.; Gangcai, C.; Yangguan, C. The econometric assessment of losses by water pollution in chongging. South West China. Chinese J. Geochemical, 2004, 23(1), 94-100.

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