Related Information

Follow us

Biological Sciences, Vol. 2, Issue 1, Feb  2018, Pages 9-18; DOI: 10.31058/j.bs.2018.21002 10.31058/j.bs.2018.21002

Production and Immobilization of Lactobacillus Acidophilus on Fruit Wastes Containing Media as Probiotics

Biological Sciences, Vol. 2, Issue 1, Feb  2018, Pages 9-18.

DOI: 10.31058/j.bs.2018.21002

Dr. Ashwini A. Waoo *1 , Shubhangi Dixit 1

1 Department of Biotechnology, Faculty of Life Sciences, AKS University, Satna, India

Received: 31 December 2017; Accepted: 25 January 2018; Published: 15 March 2018

Download PDF | XML   | Views 374 | Download 224

Abstract

The exponentially increasing world population creates the challenge of providing adequate food sources. Specially, protein deficiency poses a problem since essential amino acids cannot be replaced by anything. The threat of rising protein deficiency diseases was becoming a disaster for humans. It is essential to do some efforts to explore alternative and unconventional sources proteins from human beings and animals. Recently, the attention had drawn to exploit microorganisms directly as new source of food and feed in the form of dried cells, terms as Single Cell Protein (SCP). On the other hand, there is a great monster of waste, engulfing the whole world at an over increasing rate. Organic wastes such as vegetable and fruit residues management are also a major threat area of research concern. It is imperative to use these waste cheap substrates for culturing microbes as a source of SCP. Present research focused on attempting, production of SCP/Probiotics by Lactobacillus acidophilus. They were grown in medium containing 5g/l (NH2)2SO4, 1g/l MgSO4.7H2O, 0.5g/l NaCl, 0.1g/l CaCl2 and fruit waste as a carbon source and incubated for 7 days. The fruit wastes of Orange and Pomegranate were used as an additional supplementary element in production medium. An immobilization approach was used to store the biomass produced.

Keywords

Lactobacillus, Organic Waste, Population, Protein Deficiency, SCP

Copyright

© 2017 by the authors. Licensee International Technology and Science Publications (UK). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

[1] Moeini, H.; Nahvi L.; Tavassoli, M. Improvement of SCP production and BOD removal of whey with mixed yeast culture. ELECTRON J BIOTECHN 2004, Vol.7, No.3, pp252-258 DOI: 10.2225.
[2] Mondal, A. K.; Sengupta, S.; Bhowal, J.; Bhattacharya, D. K. Utilization of Fruit Wastes in Producing Single Cell Protein, International Journal of Science, Environment and Technology 2012, Vol. 1, No 5, pp430 – 438.
[3] Ojokoh, A. O.; Uzeh, R. E. Production of Saccharomyces cerevisiae biomass in papaya extract medium, AFR J BIOTECHNOL 2005, Vol. 4 (11), pp. 1281-1284.
[4] Khan, M.; Khan, S. S.; Ahmed, Z.; Tanveer, A. Production of Single Cell Protein from Saccharomyces cerevisiae by utilizing Fruit Wastes, Nanobiotechnica Universale 2010, Vol. 1(2), pp127-132.
[5] Singh, J. K.; Meshra, R. L.; Ramteke, D. S. Production of Single cell protein and removal of ‘COD’ from dairy waste water, European Journal of Experimental Biology 2011, Vol. 1 (3), pp 209-215.
[6] Potnis, S. S.; Mashal, B. S.; Bhartal, S. R.; Patil, D. S.; Gajbhar S. S.; Chakre G. D. Single Cell Protein production by Probiotics Grown on Fruit Waste, International Journal of Advanced Biotechnology and Research (IJBR) 2016, Vol-7, Issue-1, pp112-117.
[7] Pyar, H.; Liong, M. T.; Peh, K. K. Potentials of Pineapple Waste as Growth Medium for Lactobacillus Species, International Journal of Pharmacy and Pharmaceutical Sciences 2014, Vol 6, Issue 1, pp 142-145.
[8] Sumarno, L; Mangunwidjaja, D.; Fauzi, A. M.; Syamsu, K.; Indrasti, N. S. Production of Omega 6 Probiotic by Isolation and Fermentation of Lactobacillus plantarum JR64. Internat. J. of Waste Resources 2012, Vol. 2(1), pp11-16.
[9] Wohlgemuth, S.; Loh, G.; Blaut, M. Recent developments and perspectives in the investigation of probiotic effects. International Journal of Medical Microbiology 2010, v. 300, pp 3-10.
[10] Oelschlaeger, T. A. Mechanisms of probiotic actions - A review. International Journal of Medical Microbiology 2010, v. 300, pp 57-62.
[11] Kumar, A.; Kumar, D.; Isolation and characterization of bacteria from dairy samples of Solan in Himachal Pradesh for identification of Lactobacillus sp.; Int. J. Pharm. Sci. Rev. Res., 2014, 25(2), Mar – Apr; Article No. 21, Pages: 110-114.
[12] N. Vishnu and R. Sumathi; Isolation of fresh water microalgae Chlorella sp and its antimicrobial activity on selected pathogens; International Journal of Advanced Research in Biological Sciences, Res.Biol.Sci.2014; 1(3): 36-43.
[13] Singh, J.K.; Meshram R.L. and Ramteke, D. S; Production of Single cell protein and removal of ‘COD’ from dairy waste water Pelagia Research Library; European Journal of Experimental Biology. 2011, 1 (3):209-215 ISSN: 2248 –9215.
[14] Khan, M.; Khan S. S.; Ahmed, Z. and Tanveer A.; Production of Single Cell Protein from Saccharomyces cerevisiae by utilizing Fruit Wastes. Nanobiotechnica Universale Vol. 1(2), 127-132 (2010).
[15] Gour, S; Nupur, M; Singh A and Bhatnagar P; Single Cell Protein Production: A Review; International journal of current microbiology and Applied sciences, ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 251-262.
[16] Sadiq Azam, S.; Khan, Z.; Ahmad, B.; Khan I.; Ali, J.; Production of Single Cell Protein from Orange Peels Using Aspergillus niger and Saccharomyces cerevisiae; Global Journal of Biotechnology & Biochemistry, 2014, 9 (1): 14-18.
[17] Goyal, R.; Dhingra, H.; Bajpai P.; Joshi, N.; Characterization of the Lactobacillus isolated from different curd samples; African Journal of Biotechnology, 2012, Vol. 11(79), pp. 14448-14452.
[18] Mithun, S.; Dipak V.; Sheela, S.; Isolation and Identification of lactobacilli from raw milk samples obtained from Aarey Milk Colony; International Journal of Scientific and Research Publications, 2015, Volume 5, Issue 4, April 1 ISSN 2250-3153.
[19] Lebeer, S.; Vanderleyden, J.; Sigrid C. J.; Keersmaecker, J.; Genes and Molecules of Lactobacilli Supporting Probiotic Action; Microbiology and Molecular Biology Reviews, 2008, Vol. 72, No. 4, p. 728–764.
[20] Vasiee, A. R.; Yazdi, T.; Mortazavi, A.; Edalatian, M. R.; Isolation, identification and characterization of probiotic Lactobacilli spp. from Tarkhineh; International Food Research Journal, 2014, 21(6): 2487-2492.
[21] Kourkoutas, Y.; Xolias, V.; Kallis, M.; Bezirtzoglou, M.; Kanellaki, M.; Lactobacillus casei cell immobilization on fruit pieces for probiotic additive, fermented milk and lactic acid production, Process Biochemistry, 2005, 40 411–416.